Thanks for you reply, I have follow your steps and tested on Virtual system outlook is configured correctly , but on my internal network clients are unable to connect with outlook.
May be there is some internal rules problem. Really i am stuck please help..
I have create a rule to allow Outbound Mail like below
In the left panel, select Firewall Policy.
Right click, select New | Access Rule.
Name it similar to "Allow Outbound Mail".
Click Next, Allow.
Click Next, Select IMAP4, IMAPS, POP3, POP3S, SMTP, SMTPS.
Click Next, Select Internal and VPN Clients.
Click Next, Select External network.
Click Next for All Users.
But still have problem.. Please give u r email so i can forward screen shots of server.