I created an iSCSI virtual disk and target. Everything was working fine and now for some reason it says Virtual Disks Status Not Connected and Target Status Not Connected.

I tried to removing and importing back, still no connection. I am unable to connect after reboot. I tried creating a new one and still no connection. I cannot seem to figure out what happened. No one has touched this server for months. Is there a firewall rule that may have been triggered?

What is the gear you connect to ? As I would check the iSCSI initiator name in case it changed, or if you use MSCHAP, if a password got changed.